Fig. 6.

PIP₂ regulation of NMDAR currents involves actin and the major depolymerizing factor cofilin. A, C, and E, plot of normalized peak NMDAR currents showing the effect of PAO (10 μM) in neurons treated with the actin destabilizer latrunculin B (5 μM, 30 min; A), or dialyzed with the actin stabilizer phalloidin (10 μM; C), or dialyzed with the cofilin antibody (50 μM; E). The heat-inactivated antibody was used as a control. Inset, representative current traces (at time points denoted by #). Scale bars, 0.25 nA, 0.5 s. B, D, and F, cumulative data (mean ± S.E.) showing the percentage reduction of NMDAR currents by PAO in the presence of various agents. ∗, p < 0.001, t test.