Skip to main content
. Author manuscript; available in PMC: 2010 Nov 1.
Published in final edited form as: Differentiation. 2009 Jun 11;78(4):205–212. doi: 10.1016/j.diff.2009.05.002

Figure 7.

Figure 7

Treatment with the HDAC inhibitor TSA allows MyoD expression and differentiation in myoblasts expressing dnDR5. Cells were plated at 4×105 and the next day treated with TSA (100 ng/ml). In (A) at the indicated times, whole cell protein lysates were prepared and subjected to SDS-PAGE. In (B) cells were treated with TSA and cultured for 72 hours in GM as in (A). Cells were then cultured in DM for 48 hours. Lysates were prepared and subjected to SDS-PAGE. Western analysis was performed using anti-MyoD, anti-MHC or anti-β-actin and visualized by autoradiography as described in Materials and Methods. Shown are the results of one experiment that are representative of four independent experiments for (A) and two independent experiments for (B).