Table 1.

Gli3 primers for DGGE

	Primer sequence	Annealing Temp (°C)	Product size (bp)
Promoter 1 F	*AGCTTGCAGTTCCCTTGC	60	684
R	GTCCGACAATTTCTAACATCGA
Promoter 2 F	*GCCTTCGATGTTAGAAATTGTCG	62	668
R	TGGGCTGCTGGTAATCCCTGTGC
Exon 1 F	*TTTTGGAAAGTTGATGGCTCT	60	229
R	GGCTGCTGGTAATCCCTGT
Exon 2 F	*AATTGCTCCTTAAAGTAGTT	60	335
R	CATAGCTCCTGAACAAGTG
Exon 3 F	*CAATGTTGCTTTGTGAAT	60	234
R	TAAAAGCCAGCATCTCGT
Exon 4 F	*CCCCTTGTATCTGGTTTT	57	316
R	GTCTACTTTATACACGTCCC
Exon5 F	*ATTGCTGATGTGGGTTGT	60	297
R	GTTGCCTTTGCCATTTCC
Exon 6 F	*TAGGCAAGTAGCAATAAATAG	60	303
R	ACATAATGGATTCAGGAAAA
Exon 7 F	*GCTCAGCGTTTAAGTGAT	61	268
R	GCATCGACCTGTCCCTCT
Exon 8 F	*GGGATTGGAGAATTATCAG	60	299
R	AGTCTTGGGAGGAGTGGG
Exon13 F	*TTCCTTTCCACTTGACCCC	60	391
R	AAAACCCTGAGCAGATGCA
Exon 14-1 F	*TGTGAGGCAGGCAATGTG	53	325
R	GGAGAAGCAGGGCGAGAT
Exon 14-2 F	*ATCTCGCCCTGCTTCTCC	55	489
R	GCTGCTGAGGCTGCTGAA
Exon 14-3 F	*AGAGGATGAGCCTGAAGACG	56	486
R	GCTGCTCGTACCCTGCTT
Exon 14-4 F	*CTGATGCCAACCTGAACG	56	479
R	TCCACTGGTGCCACTTCC
Exon 14-5 F	*TGGTCGTCCACCCGCAGAA	58	490
R	GGCCCTTGGTAGATGTTGATGT
Exon 14-6 F	*AGATGCTTGGGCAGATTAG	60	350
R	TGAACCAGCTTTCGTGTC
Exon 14-7 F	*CGCTGTGCTCTAATCTGC	60	491
R	TATTGATTTCCGTTGGTTG

*GC clamp was added at the 5' end of the forward primers

GC clamp: cgcccgccgcgccccgcgcccggcccgccgcccccgcccg