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. 2009 Nov 19;9:402. doi: 10.1186/1471-2407-9-402

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Copyright ©2009 Denzel et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Visualisation of EpCAM cleavage dependence on cell-to-cell contact. (A) HCT8 cells were seeded at three different confluencies (D1-3), which resulted in single cells (D1), 20% (D2), and 80% confluency (D3). Thereafter, HCT8 cells were stained with antibodies specific for EpEX (green) and EpICD (red), and cellular DNA was stained with DAPI (blue). Merged pictures are displayed in the lower right quadrant of each section. Fluorescence was recorded with a laser scanning confocal microscope. Shown are three representative sections of each condition. (B) EpICD localisations were assessed semi-quantitatively and are given as percentages of cells with no (-), weak (+), intermediate (++), and strong (+++) staining at the membrane, at cell-to-cell contacts, and within the nucleus.