Figure 3.
Acid-1Y self-association monitored by analytical ultracentrifugation (AU) (150 μM Acid-1Y) and fit to monomer-n-mer equilibria where n = 8.4. Samples were prepared in 10 mM NaH2PO4, 200 mM NaCl (pH 7.1) and centrifuged to equilibrium at 25 °C at speeds of 42 (red), 50 (green), or 60 (blue) kRPM. The experimental data points are shown as open circles; lines indicate a fit to a monomer–octamer model as described in the experimental section (see Supporting Information).