Table 2.
Quantitative analysis of PAO1 biofilm formed in the presence of cyclic-di-GMP and its analogs
| Materials | Biomass (μm3 μm−2) | Average thickness (μm) | Maximum thickness (μm) | Roughness coefficient | Surface/volume ratio |
|---|---|---|---|---|---|
| c-di-GMP | 0.444 ± 0.193*** | 0.223 ± 0.158*** | 5.400 ± 0.548*** | 1.681 ± 0.191 | 3.303 ± 0.206 |
| c-GpGps | 0.453 ± 0.125*** | 0.273 ± 0.131*** | 4.600 ± 1.140*** | 1.677 ± 0.095 | 3.549 ± 0.240 |
| c-GpAp | 1.054 ± 0.595* | 0.773 ± 0.493** | 6.400 ± 1.140*** | 1.298 ± 0.354 | 2.858 ± 0.356 |
| c-GpIp | 2.618 ± 0.451* | 2.343 ± 0.570 | 11.167 ± 2.483** | 0.638 ± 0.091 | 2.105 ± 0.125 |
| No treatment | 2.098 ± 0.297 | 1.944 ± 0.438 | 16.200 ± 0.837 | 0.967 ± 0.095 | 2.300 ± 0.107 |
The analysis of image stacks was conducted using the comstat program. Results represent the mean ± SD and P-values refer to comparison with no treatment biofilm.
*
P<0.05.
**
P<0.01.
***
P<0.001.