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. 2009 Feb 19;72(1):41–52. doi: 10.1111/j.1365-2958.2009.06612.x

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Journal compilation © 2009 Blackwell Publishing

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Fig. 4 — Expression of M. smegmatis PknG during in vitro growth. A. Lysates from M. bovis BCG (lane 1), M. bovis BCGΔpknG (lane 2), M. smegmatis (lane 3), M. smegmatis-pknG^BCG (lane 4), M. smegmatis-pknG^smeg (lane 5) were separated on a 10% SDS-PAGE gel and immunoblotted using anti-PknG^BCG antiserum (right). The total protein pattern was analysed by Ponceau Red staining (left). B and C. M. smegmatis was grown in 7H9-OADC medium until stationary phase. Bacterial samples were collected at different time points (arrows in B) and analysed for the presence of PknG by immunoblotting as under A (C). BCG lysate was loaded in parallel as a positive control (lane 1). Again, the total protein pattern was analysed by Ponceau Red staining (left). Results are representative data from one experiment.