Fig. 2.
Growth characteristics and biochemical analysis of cDKO cells in vitro. A. The growth of the cDKO cell line in HMI9-T media was monitored in the presence (closed circles) and absence of tetracycline (open circles, dashed line). The arrow indicates the point where the viable cells are no longer recoverable when subcultured for up to 10 days in medium containing tetracycline. B. Immunoblots of cell extracts of WT, SKO and cDKO (plus and minus tetracycline) cells were probed with antiserum to T. brucei TRYS and to T. brucei PTR1 as a control (1 × 107 parasites in each lane). C. Intracellular T[SH]2 (closed circles) and GSH (open circles) levels in cDKO cells following the removal of tetracycline from cultures. Initial levels of T[SH]2 and GSH in untreated cells were 0.42 and 0.54 nmol(108 cells)−1 respectively. Each data point represents the means ± standard deviations from triplicate determinations.