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. Author manuscript; available in PMC: 2009 Nov 29.
Published in final edited form as: Cancer Res. 2008 Nov 15;68(22):9384–9393. doi: 10.1158/0008-5472.CAN-08-2655

Figure 3.

Figure 3

Smac mimetics induce TNFα-dependent apoptosis and cIAP-1 degradation. A, MDA-MB-231 cells were pretreated with or without TNFα-or TRAIL- blocking antibody, followed by treatment with Smac mimetics. Cell viability was determined with trypan blue assay. B, HCT116 colon cancer cells were treated with SM-164 alone, TNFα alone, or the combination for 48 h. Cell growth inhibition was determined by a WST assay. C and D, MDA-MB-231 cells were treated with Smac mimetics as indicated and the levels of cIAP-1 and XIAP were examined by Western blotting. E, MDA-MB-231 or HCT116 cells treated with or without MG-132, followed by the treatment with 100 nmol/L of SM-122 or SM-164. The levels of cIAP-1 were examined by Western blotting.