Skip to main content
. Author manuscript; available in PMC: 2010 Nov 25.
Published in final edited form as: Mol Cell. 2009 Nov 25;36(4):547–559. doi: 10.1016/j.molcel.2009.09.034

Fig. 5. ERK2 directly binds to CK2α through its docking groove.

Fig. 5

Immunoprecipitation and immunoblotting analyses were performed with the indicated antibodies.

(A) Immobilized purified His-CK2α was mixed with purified ERK2 followed by washing three times with cell lysis buffer and immunoblotting analysis. Control, nickel-agarose beads.

(B) 293T cells were transfected with a control vector or vectors expressing MEK1 Q56P and FLAG-ERK2.

(C) A vector expressing MEK1 Q56P were co-transfected with FLAG-WT ERK2, FLAG-ERK2 T/E (T157/158E), FLAG-ERK2 D/N (D316/319N), or FLAG-ERK2 T/E-D/N mutant into 293T cells.