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. Author manuscript; available in PMC: 2010 Jul 1.
Published in final edited form as: Mol Microbiol. 2009 Jun 8;73(2):141–151. doi: 10.1111/j.1365-2958.2009.06751.x

Table 1.

T4S systems discussed at the workshop

Organism/plasmid Name Function Known substrates Main new results presented1
a) T4S systems involved in animal cell infection (chromosomally encoded)
Anaplasma spp VirB/D4 Infection of macrophages AnkA, Ats-1 New substrate locating in mitochondria
Immunogenicity of T4S proteins
Bartonella henselae Trw Adhesion to erythrocytes None detected Role in host specificity
VirB/D4 Infection of endothelial cells BepA-G Role in host adaptation
Brucella spp. VirB Infection of macrophages VceA, VceC First T4S substrates detected
VirB2 interaction with host cell surface
Metabolic regulation of virB expression
Coxiella burnetii Dot/Icm Infection of phagocytes Multiple effectors Novel substrates identified
Ehrlichia chaffensis VirB/D4 Infection of macrophages None Regulation of expression, Vir subassemblies
Helicobacter pylori Cag Infection of gastric cells CagA, peptidoglycan T4S contact with β1 integrins
Legionella pneumophila Dot/Icm Infection of macrophages DNA, multiple effectors Polar secretion of substrates
Temporal/spatial distribution of effectors
b) T4S systems involved in DNA transfer (present in plasmids or genomic islands)
Agrobacterium tumefaciens pTi VirB/D4 T-DNA transfer to plants T-DNA, VirD2, VirD5, VirE2, VirE3, VirF VirB10 structure/function analysis
T4S helical localization pattern
Escherichia coli pKM101 Tra Conjugative plasmid transfer DNA, TraI2 3D structure of core complex
Escherichia coli R1 Tra Conjugative plasmid transfer DNA, TraI2 Helicase loading at relaxosome
Escherichia coli R388 Trw Conjugative plasmid transfer DNA, TrwC Transfer through Bartonella T4SS
Haemophilus influenzae GI Tfc GI horizontal transfer DNA New family of T4S systems
Neisseria gonorrhoeae GI Tra DNA secretion and transfer DNA, TraI2 Search for oriT; role of pilin
Streptococcus pIP501 Tra Conjugative plasmid transfer DNA, TraA2 Protein-protein interactions
1

See text for details and labs reporting the results.

2

Transport of the relaxase protein is assumed based on homology with other DNA transfer systems