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. Author manuscript; available in PMC: 2009 Nov 30.
Published in final edited form as: Circ Res. 2008 May 22;102(12):1502–1511. doi: 10.1161/CIRCRESAHA.107.170357

Figure 2. Leupaxin associates with FAK in huCSMC.

Figure 2

A HuCSMC cultured in 10% serum were co-stained with anti-leupaxin Ab, phalloidin, and DAPI (40 X) B. huCSMC lysates were incubated with GST or GST-FAK C-terminal fusion proteins (GST-CTI or GST-CTII) prior to Western analysis for paxillin (Pax) or leupaxin (Leu). C. GFP-tagged leupaxin was co-expressed in Cos7 cells with or without Flag-tagged FAK and immunoprecipitations (IP) with anti-Flag antibody were performed followed by immunoblotting with indicated antibodies. A 10% loading control (LC) is shown. D. Cos7 expressing Flag-FAK were incubated with GST or GST-Leu (full-length) or a N-terminal leupaxin fusion protein containing LD motifs 1-4 (GST-LD1-4) prior to Western analysis for FAK. E. Cos7 expressing GFP-Leupaxin variants with individual deletions of LD motifs 1-4 were incubated with GST or GST-CTII prior to Western analysis for GFP.