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. Author manuscript; available in PMC: 2009 Nov 30.
Published in final edited form as: Chem Biol. 2007 Nov;14(11):1221–1231. doi: 10.1016/j.chembiol.2007.10.005

Figure 5.

Figure 5

SNAPF is activated upon incubation with MPO+ macrophages stimulated with PMA, detectable by fluorescence microscopy. BM-derived macrophages from h-MPOTg and WT mice, or human MPOhi or MPOlo macrophages were incubated with 10 μM SNAPF for ten minutes prior to stimulation with 400 nM PMA. Images were acquired 30 minutes post-stimulation, using an APC filter set. a) h-MPOTg cells pre-stimulation. b) h-MPOTg cells post-stimulation. c) h-MPOTg cells co-incubated with 2 μM MPO inhibitor, post-stimulation. d) and e), WT cells pre- and post-stimulation. f) and g), MPOhi cells pre-and post stimulation, h), MPOhi cells post stimulation co-incubated with MPO inhibitor. i) and j), MPOlo cells pre-and post-stimulation. Results are representative of at least three separate experiments.