Figure 2.

Specificity of the ACTH antibody. A, ACTH-IR in a brain slice containing the arcuate nucleus from a wild-type C57BL/6J mouse visualized with diaminobenzidine and imaged with bright-field microscopy. B, Preabsorption of the ACTH antibody with ACTH 1-39 peptide eliminates immunoreactivity. C, ACTH-IR in the arcuate nucleus of a wild-type mouse and a POMC knock-out mouse (D). Immunofluorescent detection of ACTH (E) and α-MSH (F) in the same brain section shows overlap of the two immunoreactive peptides (G), although α-MSH-IR is predominately in fibers. R, Rostral; C, caudal; D, dorsal; V, ventral.