Skip to main content
. Author manuscript; available in PMC: 2010 Nov 3.
Published in final edited form as: FEBS Lett. 2009 Oct 4;583(21):3419–3424. doi: 10.1016/j.febslet.2009.09.054

Figure 4.

Figure 4

Cx40.8 does not readily form gap junction channels when transfected alone. (A,B) An example of positive dye transfer in Cx40.8-EGFP transfected cell pairs. (C,D) An example of failure of dye transfer in Cx40.8-EGFP transfected cell pairs. Arrows point to plasma membranes of adjacent cells. (E) An example of positive dye transfer in HeLa cells co-transfected with Cx40.8-EGFP and Cx43-mAPPLE. Red and green images were overlaid, and the red image is slightly underexposed (compared to B and D) to insure visualization of the yellow gap junction plaque containing both connexins (arrow). (F) Quantitative analysis of positive dye transfer in singly-transfected Cx40.8-EGFP HeLa cells and doubly transfected HeLa cells. The data sets are significantly different (p < 0.001).