Figure 5.

Flavopiridol prevents neuronal death after 24 h of reperfusion. (A and B) Representative sections of core infarct region stained for TUNEL from vehicle-treated (A) or flavopiridol-treated (500 μM) (B) animals 24 h after reperfusion. (The black bar represents 100 μm.) (C) Quantitation of TUNEL-positive cells in the ipsilateral cortical hemisphere of animals treated with vehicle or flavopiridol. Each data point represents a mean ± SEM (n = 4) and is expressed relative to the number of TUNEL-positive cells present in vehicle-treated ischemic animals at 24 h. * indicates significance (P < 0.01 as determined by independent t test). (D) H&E analyses of sections from the core infarct area of vehicle-treated or flavopiridol-treated (500 μM) animals 24 h after reperfusion. (The black bar represents 50 μm.)