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. Author manuscript; available in PMC: 2009 Nov 30.
Published in final edited form as: Ann Neurol. 2009 Sep;66(3):323–331. doi: 10.1002/ana.21738

Figure 3.

Figure 3

GTP cyclohydrolase (GTPCH) expression and activity in brain increases during perinatal period. A, RT-PCR analysis of GTPCH in fetal brain and liver homogenates at gestational 22, 25 and 29-days (E22, E25, E29). B, Western blot analysis of GTPCH protein levels in fetal brain and liver showing increased protein expression with age; β-actin levels were used as loading control. C, GTPCH densitometry indicates that E22 brains contain the lowest level of protein. D, GTPCH enzyme activity in the fetal brain shows a significant difference between E22 and E25 with an increase of ~50% which is maintained at later times. Values represent the mean±SE (n=4). (*) p<0.05 with respect to all other ages.