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Thalamus is the region of the brain with highest tetrahydrobiopterin levels. Tetrahydrobiopterin (BH4) was directly measured in parts of the brain between E22 and E32 (postnatal day 1). A, Low BH4 was found in all E22 with the thalamus showing significantly higher amounts with respect to all other brain regions. Values are mean±SE (n≥5), (*) different from E22 values; p<0.05. B, Map depicting average concentration of BH4 increases with age.
