. Author manuscript; available in PMC: 2009 Nov 30.

Published in final edited form as: Proteomics. 2009 May;9(9):2555–2567. doi: 10.1002/pmic.200800775

Table 2.

Sialic acid content and percentages of sialylated glycan of hPC and tg-PC.

	Expression level [mg/L]	Neu5Ac [mol/mol protein]^a)	Neu5Gc [mol/mol protein]	% of disialylated glycan^b)	% of total sialylated glycan^c)
hPC	-	7.4±0.3	ND	87	99
tg-PC	1000~1600	4.9±0.2	ND	12	99
[pig 110-3]	1000~1600	4.9±0.2	ND	12	99
tg-PC	100~300	4.1±0.4	ND	7	98
[pig 110-1]	100~300	4.1±0.4	ND	7	98

The concentration of PC samples was obtained by measuring OD_280nm using an extinction coefficient of 1.45 ml/mg-cm [1].

^a)

The molar ratio of Neu5Ac to Protein C was calculated from RP-HPLC analysis of sialic acid (run in triplicate).

^{b), c)}

The percentages were obtained from NP-HPLC profiles.

ND: not detectable