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N-glycan site occupancy of hPC and tg-PC.
| N-glycan site |
peptide sequence | protease used | occupancy, % | ||
|---|---|---|---|---|---|
| hPC | tg-PC [110-3] |
tg-PC [110-1] |
|||
| Asn-97 | VSFL(N)CSLDNGGCTHYCLEE | GluC+LysC | full | full | full |
| Asn-248 | EVFVHP(N)YSK | LysC | full | full | full |
| Asn-313 | R(N)RTFVLNFIK | LysC | full | full | full |
| Asn-329 | IPVVPH(N)E | GluC+LysC | 78.4±0.5a) | 77.0±1.3a) | 74.3±0.9a) |
The parentheses indicate the N-glycan sites.
The data are from the average of triplicate digests and duplicate injections.
