Table 3.
List of primers used for real time PCR and their GenBank Accession numbers
| Transcript | 5'primer | 3'primer | fragment length (bp) | Accession no. |
|---|---|---|---|---|
| Calcium binding protein (CaBP) | 5'-TTTATCATGGATCTATTGTGGGAAC-3' | 5'-CGTGATTATGCAACATCCTGTG-3' | 410 | GH716778 |
| Chitinase | 5’-ACTTCCCTTGTCCACATGTCTGTCC-3’ | 5’-TGATCAAAGCAGGGGCTCAGGT-3’ | 204 | GH716922 |
| Cathepsin B1 | 5'-AGCAACACCATTCCACATC-3' | 5'-ATAGCCTCCGTTACATCC-3' | 420 | EU035711 |
| Cytidine deaminase (CDA) | 5’-TCCCAAACTCTAAGAGAA-3’ | 5’-CGAGGTACATTAGTCCAT-3’ | 54 | DQ117977 |
| DEC_1 | 5’-ATACACGAAGTGTGGCTTGCATGTG-3’ | 5’-ACTACGCCAGGACCCATACCTTCA-3’ | 451 | GH717041 |
| Fas | 5’-GAAATATCATTCATTCTGCCCCGG-3’ | 5’-CGACGCCAAAAGTCAGCTCACTT-3’ | 159 | GH716733 |
| Ferritin3 | 5'-CTCTCCCACACTGTACCTATC-3' | 5'-CGGTCTGCATCTCGTTTTC-3' | 236 | GH716790 |
| Fibrinogen (Fg) C-terminal domain | 5’-AACCGTTAAGATTGACGAAGCCACA-3’ | 5’-GAAAGAGGCACTGCCTTCAACTTCA’3’ | 235 | GH716248 |
| Glutathione S-transferase (GST) | 5'-CAGCTGTTGGTCAACAATTTG-3' | 5'-CGAGCTAAATACGTTGCTATAGC-3' | 151 | GH716560 |
| Heat shock protein 40 (HSP-40) | 5'-GTTCCTTCTGGAGCAGATGA-3' | 5'-AAACCCCATGCTAAGGTCTC-3' | 152 | GH716707 |
| Heat shock protein 70 (HSP-70)3 | 5'-AGGCGTCGACATTCAGGTCTA-3' | 5'-TGGTGATGTTGTTGGTTTTACCA-3' | 199 | L44127 |
| Immunoglobulin (Ig) | 5’-AAAGACCTCTTACTGCGGCTCGAC-3’ | 5’-CATTTGTTTCACGTGCTTGGCTG-3’ | 219 | GH716944 |
| Low density lipoprotein receptor (LDLR) | 5’-ACATCCCAGTTCATCTGAAGCGTCT-3’ | 5’-ATTGCCACAAACAGGAGTGCACTG-3’ | 350 | GH716919 |
| C-type lectin | 5’-ACTTGGGACAGGGTTGAATGAAGG-3’ | 5’-TTTCAGGAGGGGTCACAATTTTGAA-3’ | 214 | GH716225 |
| Macrophage expressed gene 1 (Mpeg1) | 5’-TCCAAATGGAACTGATGACAACGC-3’ | 5’-TTGGTGTGTTGCAACTGAGCGAG-3’ | 199 | GH716015 |
| Melanogenic peroxidase | 5’-TCTTCAACGAATGGGTCAACATCG-3’ | 5’-AGATCGTTGGTTCTTCACCCACAGA-3’ | 213 | GH716074 |
| Mucin | 5'-GCCCCTGCACCCTGTTT-3' | 5'-ATGAGCTTCCACACACTGAGAAGT-3' | 110 | CAC83675.1 |
| Ornithine decarboxylase | 5'-AGGAATGTCGTCTGCCTGAGA-3' | 5'-TTGATTTCATCCCAGATATCTTGACT | 150 | GH716341 |
| Protein kinase C receptor (PKCR) | 5’-TTGATGCTCTTGTCACGAGATGCA-3’ | 5’-CATGGCCATGGACATTTTGTTAGTG-3’ | 212 | GH716153 |
| Serine protease | 5'-GTGAAGAATTTGGCGGGAGA-3' | 5'-GCGCTGAAGTGGACGATCA-3' | 239 | GH716785 |
| Theromacin | 5'-CGGGATGTTTCGAAGATTGG-3' | 5'-CGTTGTGGACAATTTAGACTTGGT-3' | 150 | GH716927 |
1,2
with the exception of Cathepsin B, Ferritin and HSP-70 regulation (Myers et al., 2008 and Ittiprasert et al., 2009)
3
with the exception of Ferritin (manuscript in preparation)