FIGURE 7.

Effect of O-phenanthroline on the ADP-ribosyltransferase activity of NarE mutants. NarE mutants were tested for their ability to transfer ADP-ribose moiety and release nicotinamide with (white bars) and without (black bars) 10 mm O-phenanthroline. ADP-ribosyltransferase activity was measured for 18 h at 30 °C under the same conditions of previous experiments and as described under “Experimental Procedures.” Data are expressed as ADP-ribosylagmatine formed per mg of purified protein. Error bars represent the S.D. of three independent experiments.