TABLE 1.

Steady state rate of the Srx-catalyzed reaction with wild-type, C48S, C106A, and C48A/C106A Srx

Reactions were carried out in buffer TK at 30 °C with 1 mm ATP, 1 mm MgCl₂, 100 μm C171A Prx, and 5–10 μm Srx. The reactions were followed with Trx (50 μm) by the Trx/NTR assay and with DTT (50 mm) by the purine nucleoside phosphorylase assay.

Reductant	Wild type	C106A	C48S	C48A/C106A
Trx	1.7 ± 0.2	1.9 ± 0.3	0.18 ± 0.2	0.25 ± 0.7
DTT	2.2 ± 0.4	2.1 ± 0.2	1.2 ± 0.2	1.2 ± 0.2