TABLE 1.
Bacterial strains and plasmids
| Bacterial strain or plasmid | Description | Source or reference |
|---|---|---|
| Salmonella strains | ||
| 12023 | Parental strain | (36) |
| DH215sc4 | ΔsifA | (8) |
| PH011 | ΔsifA, psifA-2HA | (12) |
| LD101 | ΔsifA, psifA(L130D)-2HA | This study |
| LD102 | ΔsifA, psifA(WXXXA)-2HA | This study |
| LD103 | ΔsifA, psifA(AXXXE)-2HA | This study |
| LD104 | ΔsifA, psifA(AXXXA)-2HA | This study |
| E. coli strains | ||
| DH5α | F− f80lacZDM15 D(lacZYA-argF)U169 endA1 recA1 hsdR17 deoR supE44 thi-1 gyrA96 relA1 | (37) |
| BL21 λ (DE3) | F−ompT rB− mB− (DE3) | (38) |
| C41 λ (DE3) | F−ompT rB− mB− (DE3) modified | Avidis |
| Plasmids | ||
| pGEX-4T2 | GST in eukaryotic cells | Pharmacia |
| pEGFP-C1 | GFP in eukaryotic cells | Clontech |
| pKH3-HA3-RhoA | 3HA::RhoA in eukaryotic cells | (39) |
| pKH3-HA3-RhoAN19 | 3HA::RhoAN19 in eukaryotic cells | (39) |
| pKH3-HA3-RhoAL63 | 3HA::RhoAL63 in eukaryotic cells | (39) |
| pGST::SKIP(PH)(762–885) | GST::SKIP(PH) in E. coli | (12) |
| pMyc::SKIP | Myc::SKIP(PH) in eukaryotic cells | (12) |
| pSifA::GFP | SifA::GFP in eukaryotic cells | This study |
| pGST::SifA | GST::SifA in E. coli | This study |
| pGFP::SKIP(PH) | GFP::SKIP(PH) in eukaryotic cells (pEGFP-C3) | This study |
| pGFP::SifA-(1–140) | GFP::SifA-(1–140) in eukaryotic cells (pEGFP-C1 derivate) | Ruiz-Albert and Holden |
| pGFP::SifA-(41–336) | GFP::SifA-(41–336) in eukaryotic cells (pEGFP-C1 derivate) | Ruiz-Albert and Holden |
| pGFP::SseJ-(1–150) | GFP::SseJ-(1–150) in eukaryotic cells (pEGFP-C1 derivate) | Ruiz-Albert and Holden |
| pMyc::SifA-(1–140) | Myc::SifA-(1–140) in eukaryotic cells | This study |
| pMyc::SifA-(41–336) | Myc::SifA-(141–336) in eukaryotic cells | This study |
| pMyc::SseJ-(1–150) | Myc::SseJ-(1–150) in eukaryotic cells | This study |
| pCMV-HA::SKIP | HA::SKIP in eukaryotic cells | This study |
| pDONR-His::SifA | His::SifA, Gateway entry clone | This study |
| pDONR-His::SKIP(PH) | SKIP(PH), Gateway entry clone | This study |
| pDONR-His::SifA(6) | SifA(s2985), Gateway entry clone | This study |
| pDONR-His::SifA(9) | SifA(s3015), Gateway entry clone | This study |
| pHis::SifA | His::SifA in E. coli | This study |
| pHis::SKIP(PH)-(771–878) | His::SKIP(PH) in E. coli | This study |
| pHis::SifA(6) | His::SifA(s2983) in E. coli | This study |
| pHis::SifA(9) | His::SifA(s3015) in E. coli | This study |
| pSifA-(1–39)::GFP | SifA-(1–39)::GFP in eukaryotic cells | This study |
| pSifA-(1–75)::GFP | SifA-(1–75)::GFP in eukaryotic cells | This study |
| pSifA-(1–105)::GFP | SifA-(1–105)::GFP in eukaryotic cells | This study |
| pSifA-(1–140)::GFP | SifA-(1–140)::GFP in eukaryotic cells | This study |
| pSifA-(36–140)::GFP | SifA-(36–140)::GFP in eukaryotic cells | This study |
| pSifA-(71–140)::GFP | SifA-(71–140)::GFP in eukaryotic cells | This study |
| pSifA-(106–140)::GFP | sifA-(106–140)::GFP in eukaryotic cells | This study |