FIGURE 7.

HDAC activity is involved in the ROS-mediated HIF-1α transcription. A, P29 and A11 cells were treated with TSA at the indicated concentrations for 18 h. Total RNA was extracted and subjected to Northern blot analysis. The blots were hybridized with a ³²P-labeled HIF-1α cDNA. Ethidium bromide staining of the gel is also shown. B, HDAC activity is shown in untreated P29 and P29 cells treated with 25 μm H₂O₂ for 16 h, and untreated A11 and A11 cells treated with ebselene (20 μm), antimycin A (20 μm), LY294002 (20 μm), Ro31-8220 (5 μm), and TSA (100 ng/ml) for 18 h.