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. 2009 Sep 30;284(48):33195–33205. doi: 10.1074/jbc.M109.020586

FIGURE 3.

FIGURE 3.

SPE B activates the JAK2/STAT1 signal pathway through αVβ3. A549 cells (2 × 105) were preincubated with AG490 (15 μm) for 1 h and then treated with SPE B (20 μg/ml) for the indicated times (A). Cells were also incubated with SPE B (20 μg/ml) or G308S (20 μg/ml) for the indicated times (B and C), and cell lysates were immunoprecipitated (IP) with anti-JAK2 antibody (A and B) or anti-αVβ3 antibody (C) and immunoblotted using anti-phosphotyrosine or anti-JAK2 antibody (A and B) and anti-JAK2 or anti-αVβ3 antibody (C). Cells were also preincubated with various concentrations of AG490 for 1 h and then treated with SPE B (20 μg/ml) or G308S (20 μg/ml) for the indicated times (D). Cells were treated with SPE B (20 μg/ml) or G308S (20 μg/ml) for the indicated times with or without anti-αVβ3 antibody (4 μg/ml) (E), cells (2 × 105) were also transfected with siRNA of JAK2 and incubated with SPE B or G308S (20 μg/ml) for 30 min (F), and cell lysates were immunoblotted using anti-phospho-Tyr-701 STAT1 or anti-STAT1 antibody.