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. 2009 Sep 30;284(48):33195–33205. doi: 10.1074/jbc.M109.020586

FIGURE 7.

FIGURE 7.

Phosphorylated STAT1 binds to procaspase 8 and Bax promoters after SPE B treatment. ChIP assay was used to detect the binding of STAT1 on the promoter of procaspase 8 or Bax. Cells (3 × 106) were treated with SPE B (20 μg/ml) with or without AG490 (15 μm) or SB203580 (10 μm) for 4 h. Control cells were treated with SPE B only. Chromatin was extracted and immunoprecipitated (IP) with antibodies specific to STAT1. Promoter of procaspase 8 (A) or Bax (D) was amplified with indicated primers by PCR. Input indicates the amount of genomic DNA used for immunoprecipitation. For the expression of mRNA, cells (3 × 106) were treated with SPE B (20 μg/ml) with or without AG490 (15 μm) or SB203580 (10 μm) for 4 h. Total RNA was prepared and analyzed by RT-PCR for procaspase 8 (B) or Bax (E) mRNA expression. Cells (2 × 105) were also transfected with siRNA of JAK2 or p38 and incubated with SPE B (20 μg/ml) for 10 h, and total cell lysates were immunoblotted using anti-procaspase 8 (C) or anti-Bax antibody (F).