FIGURE 8.

SPE B-induced cell apoptosis is associated with procaspase 8 and Bax expression. Cells (8 × 10⁴) were treated with SPE B or G308S (20 μg/ml) for the indicated times (A). Apoptosis was then determined using propidium iodide staining followed by flow cytometry analysis. Results are the mean ± S.E. of three experiments. *, p < 0.05. Cells (8 × 10⁴) were also treated with SPE B or G308S (20 μg/ml) with or without AG490 (15 μm) and SB203580 (10 μm) pretreatment for 1 h (B). Apoptosis was determined after 24 h. Results are the mean ± S.E. of three independent experiments. *, p < 0.05; **, p < 0.01; ***, p < 0.001. After transfection with various siRNAs, cells (8 × 10⁴) were treated with SPE B (20 μg/ml), and the level of apoptosis was measured after 24 h (C). Data are the mean ± S.E. of three experiments. *, p < 0.05.