FIGURE 1.

FANCF is an ICSBP-target-gene in differentiating myeloid cells. A, a schematic representation of consensus sequences for IRF-protein binding in the proximal FANCF 5′-flank is shown. The sequence of the proximal FANCF 5′-flank was analyzed for IRF DNA binding consensus sequences (underlined in red). The ICSBP binding cis element is indicated by an asterisk. The red arrow indicates the 5′ end of the sequence of the clone obtained by ICSBP co-immunoprecipitation. The black arrow indicates the transcription start site. TSS, transcription start site. B, ICSBP interacts with the proximal 5′-flank of the FANCF gene in vitro in IFNγ-differentiated, but not untreated, U937 myeloid cells. Chromatin co-immunoprecipitation (IP) was performed with U937 myeloid leukemia cells (with or without IFNγ-induced differentiation) and an antibody to ICSBP (or irrelevant control antibody). Co-precipitating chromatin was amplified with primers encompassing 0 to −1.5 kb, 0 to −0.5 bp, 0 to −150 bp, −0.5 bp to −1.5 kb, or −1.5 to −2.0 kb in the proximal FANCF 5′-flank or +1.5 to +2.0 kb (in exon I).