FIGURE 2.

A, gene expression of ATP citrate-lyase (ACL), acetyl-CoA carboxylase (ACC), FAS, malic enzyme (ME), and hepatic stearoyl-CoA desaturase 1 (SCD1), assessed by RT-qPCR in the livers of WT mice and HCV transgenic (HCV-tg) littermates (all males, 8–9 months old). The results were normalized to the mean expression level in WT liver. B, FAS protein expression, assessed by Western blotting. The left-hand panel shows a representative blot. Variations between samples were normalized on the basis of GAPDH expression. The results were normalized to the mean expression level in WT liver. Box-and-whisker graphs are used. The values in WT and HCV-tg mice were compared with the Mann and Whitney test.