TABLE 2.
Mass isotopomer distribution of 4-hydroxynonanoate (C9) and 4-hydroxypentanoate (C5) and metabolites in livers perfused with 100% 2H2O buffer
The first column lists the compounds assayed in livers perfused with C5 or C9 4-hydroxyacid. Note that the two substrates were unlabeled when added to the labeled buffer. All data are corrected for natural enrichment. Also the mass isotopomer distributions of the 4-OH-acyl-CoAs and 4-phospho-acyl-CoAs have been corrected for the incorporation of 2H in the CoA moiety. The total labeling represent the sum of the mol fractions of labeled isotopomers (M1, M2, …).
| Compound | M | M1 | M2 | M3 | M4 | M5 | M6 | M7 | M8 | M9 | Sum labeling | Total corrected labeling |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 4-OH-acid | ||||||||||||
| C5 | 72.5 | 11.4 | 10.4 | 5.5 | 0.0 | 0.0 | 0.1 | 0.0 | 0.0 | 0.0 | 27.5 | |
| C9 | 74.5 | 7.5 | 8.1 | 6.8 | 0.6 | 2.4 | 0.0 | 0.0 | 0.0 | 0.0 | 25.5 | |
| Free CoA | ||||||||||||
| C5 | 95.6 | 1.3 | 1.1 | 0.7 | 1.2 | 0.1 | 0.1 | 0.0 | 0.0 | 0.0 | 4.4 | |
| C9 | 87.5 | 4.7 | 2.6 | 1.7 | 2.9 | 0.3 | 0.2 | 0.0 | 0.0 | 0.0 | 12.5 | |
| 4-OH-acyl-CoA | ||||||||||||
| C5 | 38.6 | 23.4 | 10.5 | 10.5 | 10.4 | 6.6 | 0.0 | 0.0 | 0.0 | 0.0 | 61.4 | 57.0 |
| C9 | 57.7 | 18.7 | 9.5 | 9.3 | 4.1 | 0.5 | 0.0 | 0.3 | 0.0 | 0.0 | 42.3 | 29.8 |
| 4-P-acyl-CoA | ||||||||||||
| C5 | 13.1 | 30.1 | 13.4 | 15.1 | 15.6 | 8.8 | 2.8 | 0.8 | 0.4 | 0.0 | 86.9 | 82.5 |
| C9 | 13.1 | 14.4 | 15.0 | 18.0 | 17.1 | 10.0 | 4.8 | 2.4 | 1.4 | 1.4 | 84.5 | 72.0 |