FIGURE 1.

Domains/regions of full-length CCTα and the CCT-catalyzed reaction. A, CCTα isoform consists of four domains or regions as follows: the N-terminal region (N; 1–75) with the NLS, the catalytic domain (C; 76–236), the membrane binding domain (M; 237–314), and the phosphorylation region (P; 315–367). The domain/region boundaries are approximate. The CCT236 construct is shown as a bar, and the region revealed in the crystal structure (40–215, chain A) is in gray. B, CCT-catalyzed mechanism for CDP-choline synthesis from CTP and phosphocholine. The nucleophilic phosphocholine phosphate becomes the β*-phosphate of the product.