Table 1.
GCR rates for different positions of the CAN1/URA3 cassette on chromosome V.
| Assay | Wild-type | rad27Δ | mre11Δ | sgs1Δ | Breakpoint Region Length (kb)** |
Wild-type Rate / Length (kb-1) |
||||
|---|---|---|---|---|---|---|---|---|---|---|
| Strain RDKY# |
Rate* | Strain RDKY# |
Rate* | Strain RDKY# |
Rate* | Strain RDKY# |
Rate* | |||
| Standard GCR*** | 3615 | 3.5×10-10 (1) | 3630 | 3.7×10-7 (1057) | 3633 | 2.2×10-7 (629) | 3813 | 2.5×10-8 (71) | 11.6 | 3.0×10-11 |
| yel062w::CAN1/URA3 | 6675 | 1.15 [0.0-5.6]×10-10 (0.3) | 6679 | 6.87×10-7 (2180) | 6680 | 3.23×10-7 (496) | 6681 | 1.19×10-8 (34) | 9.7 | 1.2 [0.0-5.7]×10-11 |
| yel064c::CAN1/URA3 | 6676 | 5.09 [2.5-7.7]×10-10 (1.6) | 6682 | 7.47×10-7 (2371) | 6683 | 2.63×10-7 (465) | 6684 | 1.77×10-8 (51) | 14.5 | 3.5 [1.7-5.3]×10-11 |
| yel068c::CAN1/URA3 | 6677 | 2.27 [1.3-4.8]×10-9 (7.2) | 6685 | 5.57×10-7 (1591) | 6686 | 5.75×10-7 (1643) | 6687 | 1.69×10-8 (48) | 19.2 | 12 [6.8-25]×10-11 |
| yel072::CAN1/URA3 | 6678 | 1.97 [1.6-4.3]×10-8 (56) | 6688 | 2.78×10-6 (7943) | 6689 | 1.52×10-6 (4345) | 6690 | 1.93×10-6 (5515) | 31.0 | 64 [52-140]×10-11 |
| yel068c::CAN1/URA3 hxt13-dsf1Δ | 6872 | 1.43 [0.0-4.2]×10-9 (4.1) | 6873 | 3.74×10-7 (1068) | - | n.d. | 6874 | 4.39×10-9 (12) | 19.2 | 7.5 [0.0-22]×10-11 |
| yel072::CAN1/URA3 hxt13-dsf1Δ | 6875 | 5.64 [4.1-12]×10-9 (16) | 6876 | 5.22×10-7 (1492) | - | n.d. | 6877 | 1.17×10-8 (33) | 27.7 | 20 [15-42]×10-11 |
*
Rate of accumulating CanR 5FOARprogeny. The number in parentheses is the fold increase relative to the standard wild-type rate (3.5×10-10; 16). Numbers in brackets are the 95% confidence interval limits.
**
The breakpoint region is defined as the length between the telomeric end of PCM1 and the telomeric end of CAN1.