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Molar ratio of sulfite to oxygen for wild-type tauD and F159 variants, determined in 50 mM bis-Tris pH 6.2 containing 200 μM ascorbate. Darker bars represent values obtained using 1,1-[1H]2-N-methyltaurine, and lighter bars represent values obtained using 1,1-[2H]2-N-methyltaurine. Error bars represent 2σ deviations determined from replicate determinations (n = 3–5).
