Fig. 1.

Generation of p55^−/− mice. (A) Domain structure of the p55-like MAGUKs. Seven family members (MPP1-MPP7) have been identified in mammals. (B) Location of the p55/MPP1 gene in the q28 region of the human and mouse X chromosome. Both MPP1 and factor VIII genes are transcribed in the same orientation while the gene for X-linked dyskerotosis congenita (dyskerin/DKC1) lies tail to tail with MPP1. (C and D) Gene targeting construct for the deletion of p55/MPP1 gene. The C-terminal half of p55 was deleted including the D5, TP, and GUK domains encoded by exons 7–11. (E) Confirmation of the genotype by PCR of tail DNA, and Western blotting of p55 in the erythrocyte ghosts.