Fig. 1.

Localization of endogenous mRNAs encoding peroxins. (A) Representative fluorescence microscopy images of cells bearing the MS2L sequence integrated into different genes (as indicated; see the ORF_INTstrains in Table S2) and transformed with plasmids expressing MS2-CP fused with 3 GFP molecules [MS2-CP-GFP(x3)] and RFP-PTS1, as a marker for the peroxisomes, are shown. Cells were grown overnight on medium containing oleate and induced with the same medium lacking methionine for 1 h before visualization. The percentage of GFP-labeled RNA granules that colocalize with peroxisomes is shown. mRNA indicates labeled RNA granules; RFP-PTS1 indicates peroxisomes. (Scale bar: 2 μm). (B) Integration of the MS2 loops does not alter protein function. MS2L-integrated yeast strains (as indicated) were grown to log phase on glucose-containing medium, normalized for cell number, diluted serially, and plated by drops onto solid medium containing oleate.