Fig. 4.
Enhanced binding of hCCR4 to the DIO1 gene promoter. Shown are the results of ChIP assays for HeLa cells transfected with hCCR4 and treated with vehicle (lane 1) or Triac (10−7 M) for different times: 0 min (lane 1), 5 min (lane 2), 10 min (lane 3), 60 min (lane 4), 120 min (lane 5), 360 min (lane 6), and overnight (lane 7). Following formaldehyde cross-linking, lysates were immunoprecipitated with antibodies against hTRβ1 or pre-immune serum as indicated. Primers specific for the DIO1 promoter and the coding region of GAPDH were used to amplify the DNA associated with hTRβ1 and hCCR4 in vivo.