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. 2009 Nov 10;106(47):19878–19883. doi: 10.1073/pnas.0908458106

Fig. 1.

Fig. 1.

The expression and functional role of DAB2IP in PCa. (A) Box plot of relative DAB2IP mRNA levels from human specimens of BPH (n = 12, left bar), primary cancer (n = 12, center bar), and bone metastases (n = 12, right bar). Thin line = median; dotted line = mean; P < 0.001 by Kruskal–Wallis ANOVA on RANKS. (B) DAB2IP induced G0/G1 cell cycle arrest. Cell cycle distribution was detected by flow cytometry. For cyclins/DNA multiparameter flow cytometry, the trapezoidal “window” represented the level of fluorescence of the isotype IgG control, indicating the range within which cyclin-negative cells were located. (C) Transient expression of DAB2IP promoted apoptosis in C4-2 cells under LY treatment. Apoptosis was determined by Annexin V/PI flow cytometry. Asterisk indicates statistical significance in cells transfected with DAB2IP vs. VC (P < 0.01). (D) DAB2IP promoted apoptosis in D1 and D2 cells treated with LY or TNF-α. Asterisk indicates statistical significance between DAB2IP-expressing cells and neo cells (P < 0.01). (E) Knockdown of DAB2IP reduced the apoptotic response of PZ-HPV-7 cells. Asterisk indicates statistical significance in cells transfected with DAB2IP-siRNA vs. control siRNA (P < 0.01). All of the data (mean ± SEM) were generated from three independent experiments.