Table 1.

Molecular verification of predicted Helitrons

Inbred line	Predicted Helitrons
	Silco_101	Silico_102	Silico_103	Silico_106	Silico_107	Silico_110
4Co63	Vacant	Vacant	Occupied	Vacant	Vacant	Occupied
A188	Vacant	Occupied	Vacant	Occupied	Vacant	Occupied
BSSS53	Vacant	Vacant	Occupied	Vacant	Vacant	Occupied
B73	Occupied	Occupied	Occupied	Occupied	Occupied	Occupied
CML139	Vacant	Vacant	Occupied	Vacant	Vacant	Vacant
H99	Vacant	Occupied	Occupied	Occupied	Vacant	Occupied
I137TN	Vacant	Occupied	Occupied	Occupied	Vacant	Occupied
Ki3	Vacant	Vacant	Occupied	Vacant	Vacant	Occupied
M14	Vacant	Occupied	Occupied	Vacant	Vacant	Occupied
Mo17	Vacant	Vacant	Occupied	Occupied	Vacant	Occupied
McC	Vacant	Vacant	Occupied	Occupied	Vacant	Vacant
W22	Vacant	Vacant	Occupied	Occupied	Vacant	Vacant
W23	Occupied	Vacant	Occupied	Vacant	Vacant	Occupied

Vacant (no Helitron): PCR product obtained. Occupied (Helitron present): no PCR product. B73 is the positive control and H2O served as negative control.