Fig. 4.

HNF1 and p300 associate with multiple DHS of the CFTR locus in Caco2 cells. Real-time PCR analysis of Caco2 chromatin immunoprecipitated with indicated antibodies at various locations along the locus. Each value shown is relative to enrichment measured with isotype-matched IgG control (dotted line). Data for each antibody are from a single representative ChIP experiment (each experiment performed at least twice). All data points were calculated as percentage of input material and then normalized to background 18s rRNA levels; error bars represent SEM of at least two PCR reactions for each fragment. Black bars below graphs represent locations of Caco2 DHS (see Fig. 1).