Fig. 4.

HIV-1_BAL preferentially infects expanded Ad-specific CD4 T cells. CFSE-stained lymphocytes were co-cultured with autologous DCs that were unpulsed or pulsed with Ad5, Ad11, or second-generartion Ad5 for 3 days. Cells were then cultured in the presence of infectious HIV-1_BAL for an additional 4 days. HIV-1 infection of T helper cells was then measured by intracellular labeling of CD3⁺ CD8⁻ cells for p24 gag. The gating strategy to identify HIV-1 infected Ad-specific CD4 T cells is shown in (A) where quadrants were based on p24 stained uninfected T cells subjected to the same conditions. (B) The mean percentages of HV-1 p24 positive divided CFSE^low (green bars), undivided CFSE^high (blue bars), or total CD3⁺ CD8⁻ cells (white bars) are shown (n = 4). Error bars, SEM; P values were obtained using the Mann Whitney U test. (C) Lymphocytes from 4 Ad5-responders were either cultured alone or co-cultured with unstimulated or Ad5, Ad11, or tetanus toxoid-stimulated autologous DCs for 3 days. Cells were then infected with HIV-1_BAL for an additional 7 days. HIV-1 p24 levels were then measured in cell culture supernatants by ELISA as indicated in Materials and Methods. The means are given; error bars, SEM. P values were obtained using the Mann Whitney U test.