Fig. 2.

PAO and DTNB modify the same substrates for oxidative sensitization of TRPV1. (A) PAO activated rTRPV1 basal currents (pH 7.4), which was reversed by DTT. DTNB later reactivated receptors in the same patch, albeit less effectively (I_DTNB/I_PAO = 0.30 ± 0.03, n = 8). (B) PAO-sensitized rTRPV1 is refractory to subsequent DTNB application. (C) PAO-potentiated hTRPV1 quickly recovered its DTNB sensitivity by brief BAL exposure. In contrast, reversal of DTNB sensitization required a long application of BAL (36 min). All experiments were conducted in inside-out patches.