FIGURE 3.

The optimal length of DNA for TAF4b·TAF12 binding is half the size of nucleosomal DNA. A, the TAF4b·TAF12 complex was incubated with a radiolabeled oligonucleotide containing a 70-bp AdML promoter (WT) in the absence (lane 2) or the presence (lanes 3–10) of cold DNA competitors as indicated at the top of the lanes. The sequences of the DNA used as competitors are shown below. B, EMSA experiment as in A using equivalent molar amounts of different length DNAs whose sequences are shown in A. The relative amount of bound DNA is indicated at the bottom. C, competition experiment as in A. Sequences of competitor DNA are shown at the bottom. Mutated nucleotides are in lowercase letters.