FIGURE 3.

Schizophyllan promotes the transition of SC3 to the β-sheet state at the water-PTFE interface. WCA measurements were made on PTFE sheets coated with 5, 50, and 100 μg ml⁻¹ SC3 (pH 7) and washed with water (5 W, 50 W, and 100 W) or 0.1% Tween 20 at pH 7 (5 T, 50 T, and 100 T). Coating was performed for 2 h (white and dark gray bars) or 16 h (light gray and black bars) in the absence (white and light gray bars) or presence (dark gray and black bars) of 0.3 mg ml⁻¹ schizophyllan (SCH). The effect of schizophyllan was most clear at a low SC3 concentration. The WCAs of PTFE sheets coated at 5 μg ml⁻¹ SC3 were much lower after treatment with Tween 20 (pH 7) when the coating was performed in the presence of schizophyllan (see 5 T samples). deg., degrees.