FIGURE 4.

A, quantitative analysis of SPTLC3 mRNA expression in human and non-human cell lines. Cell lines like HEK293, THP1, or HeLa express SPTLC3 in low amounts, whereas higher expression was observed in the trophoblast cell lines JAR and Jeg3. B, correlation of SPTLC3 mRNA expression and the accumulated C₁₆-SA in FB1-treated cells. Cells were incubated with FB1 for 24 h, and the accumulated C₁₆-SA was analyzed by LS-MS. We found a good correlation between SPTLC3 mRNA expression levels and the levels of accumulated C₁₆-SA (R² = 0.98).