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The levels and phosphorylation status of eEF2 in the cortices of BDNF mutant mice. Western blotting (A) and densitometric analysis (B) were performed to examine the total and phosphorylated (inactive) protein levels of eEF2 in cortical tissues of wild-type (wt), BDNF mutant (KO(−/−)), and BDNF transgenic (Tg) mice. Bars represent the mean ± S.D.; n = 4, *, p < 0.05 (analysis of variance). The phosphorylation ratio of eEF2 was calculated and is shown in panel C. KO, knock-out.
