FIGURE 6.

Relationship of Blm and Exo1 and an ATPase-independent promotion of HR between diverged sequences by Blm. A, overexpression of either Blm or Exo1 significantly increases HR between diverged sequences. BLM and/or Exo1 transgenes together with I-SceI expression vector were transfected in wild type cells carrying S2neo with targeting-DNA-fragment Mneo-3. The error bars represent standard deviations. The data for the negative control was from B. B, human BLM (wild type and K695A) transgenes together with I-SceI expression vector were transfected in wild type cells carrying S2neo with targeting-DNA-fragment Mneo-3. Gene targeting frequency and standard deviation are also presented.