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. 2009 Jul 28;284(39):26394–26401. doi: 10.1074/jbc.M109.015875

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© 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 4. — A, half-life of ∼50-nt processing intermediate (arrow) in the RNA with the upstream NotI site (RNA A5). B, sizing of the ∼50-nt RNA on a high-resolution gel by Northern blot analysis of in vivo RNA isolated from strain carrying RNA A5 (left) and after PNPase processing in vitro (right). The in vitro samples were incubated without (−) or with (+) PNPase. C, comparison of amount of full-length (FL) and ∼50-nt processing intermediate (arrow) in A5 and A6 RNAs. D, in vitro PNPase activity on trp leader RNAs without and with the upstream NotI sequence. Sizes of full-length substrates (114 nt for wild-type, 127 nt for RNA A5) are indicated, as well as the approximate size of the PNPase digestion product for the RNA with the upstream NotI sequence. The control lane C contained no added PNPase. Above each lane is indicated time (min) after PNPase addition.