FIGURE 3.
PFA inhibition of DNA synthesis by viral polymerases under steady-state conditions for single nucleotide incorporation events. A, single nucleotide incorporation events in the presence of constant concentrations of dCTP and increasing concentrations of PFA. The migration pattern of the 5′ end radioactively labeled primer (s) and product (p) is illustrated by corresponding arrows. B, graph of data shown below A. IC50 values for PFA were calculated by fitting the data points to a sigmoidal dose response. Dotted lines illustrate the respective IC50 values for PFA.