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Reporter assay in which A293 cells were transfected with the luciferase constructs driven by the HAS1 proximal promoter containing the CRE (CRE), lacking the CRE (no CRE), and having a mutated form of CRE (CRE mut, mutated residues underlined). The cells were treated or not with 10−3 m db-cAMP. The results are expressed as relative light units, i.e. the ratio of light output:β-galactosidase activity.
